Since its birth, the real-time quantitative PCR instrument detection technology has been more and more favored by laboratory teachers. It is a method that uses fluorescent chemicals to measure the total amount of products

The primers added in the PCR amplification of the automatic gradient PCR instrument are labeled with isotopes, fluorescein, etc., and the primers and fluorescent probes are used to specifically bind to the template at the same time for amplification.

1. Isolate high-quality RNA Successful cDNA synthesis comes from high-quality RNA. High-quality RNA should be full-length and free of reverse transcriptase inhibitors such as EDTA or SDS, and minimize genomic DNA contamination.

Gradient PCR gene amplification instrument is an instrument and equipment that uses PCR (polymerase chain reaction) technology to amplify specific DNA.

A. DNA template:Try to use high-quality, purified DNA as a template· When the fidelity needs to be improved, a higher DNA template concentration can be used and the number of cycles can be reduced·