Views: 4 Author: Site Editor Publish Time: 2021-12-21 Origin: Site
Gradient PCR gene amplification instrument is an instrument and equipment that uses PCR (polymerase chain reaction) technology to amplify specific DNA. It is widely used in medical and biological laboratories, for example, to determine whether a sample will show certain Atlas of genetic diseases, diagnosis of infectious diseases, gene duplication, and paternity testing, etc.
PCR (polymerase chain reaction) is a method of enzymatically synthesizing specific DNA fragments in vitro. It consists of several steps of high temperature denaturation, low temperature annealing, and temperature extension. It has the characteristics of strong specificity, high sensitivity, simple operation and time-saving.
The main function of PCR gene amplification instrument is similar to the basic measuring instrument, and it has higher requirements for measurement elements. Once out of control, the instrument will not work normally, so the PCR gene amplification instrument also needs regular maintenance.
Problems that need attention in the maintenance of gradient PCR gene amplification instrument:
1. The PCR gene amplification instrument needs to be tested regularly, usually at least once every six months.
2. The required temperature of the PCR reaction is inconsistent with the actual distribution of the reaction temperature. When the average temperature difference of each well is found to deviate from the set temperature by more than 1 to 2°C, the temperature correction method can be used to correct the actual PCR reaction temperature difference.
3. The key to the PCR reaction process is the time control of the rising and cooling process. The shorter the requirement, the better. When the cooling process of the PCR machine exceeds 60s, the refrigeration system of the instrument should be checked, and the reaction base should be cleaned for the air-cooled PCR machine. For other refrigeration systems, check the relevant refrigeration components.
4. In general, if the temperature correction method can be used to correct the temperature of the gradient PCR gene amplification instrument, do not open or adjust the electronic control parts of the instrument easily. If necessary, please ask the maintenance personnel to repair or use the detailed drawings of the electronic circuit of the instrument for maintenance.