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PCR laboratory planning and design

Views: 17     Author: Site Editor     Publish Time: 2021-09-23      Origin: Site

Gene amplification laboratory, also known as PCR laboratory, is characterized by the ability to greatly increase trace amounts of DNA and is widely used in various fields of biology.

In principle, the PCR laboratory is divided into four separate working areas: reagent preparation area, specimen preparation area, amplification area and amplification product analysis area. In order to avoid cross-contamination, entering each work area must strictly follow the single direction principle, that is, only from reagent preparation area → specimen preparation area → amplification area → amplification product analysis area, and no reverse flow is allowed.


Basic requirements:

1. Each area must be independent of each other;

2, the equipment and various items in each area must be dedicated;

3. Each area is completely independent, and the buffer room is optional. If it is a mode of one area with one area, the area cannot be directly connected, and there must be a buffer room to ensure that the two areas are always isolated;

Reagent preparation area: for the preparation, packaging and storage of amplification reagents, the air pressure in this area should be kept slightly positive. The main equipment includes balance, refrigerator, centrifuge, sampler, oscillator, and ultraviolet lamp.

Specimen preparation area: mixing of laboratory samples and preparation of test samples. The working area is under negative pressure or decompression, and an exhaust system is installed. The main equipment includes refrigerators, biological safety cabinets, centrifuges, samplers, oscillators, constant temperature water baths, and ultraviolet lamps.

Amplification area: preparation of PCR amplification reaction system and addition of templates, nucleic acid amplification. The working area is under negative pressure or decompression, and an exhaust system is installed. The main equipment includes nucleic acid amplification equipment, refrigerators, clean benches, centrifuges, sample dispensers, and ultraviolet lamps.

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